The impact of glutathione transferase kappa deficiency on adiponectin multimerisation in vivo

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dc.contributor.authorTheodoratos, Angelo
dc.contributor.authorBlackburn, Anneke
dc.contributor.authorCoggan, Marjorie
dc.contributor.authorCappello, Jean
dc.contributor.authorLarter, Claire
dc.contributor.authorMatthaei, Klaus
dc.contributor.authorBoard, Philip
dc.date.accessioned2015-12-10T22:27:30Z
dc.date.issued2012
dc.date.updated2015-12-09T09:41:22Z
dc.description.abstractGlutathione transferase Kappa (GSTK1-1) also termed disulfide bond-forming oxidoreductase A-like protein (DsbA-L) has been implicated in the post-translational multimerization of adiponectin and has been negatively correlated with obesity in mice and humans. We investigated adiponectin in Gstk1-/- mice and surprisingly found no difference in the levels of total serum adiponectin or the level of high molecular weight (HMW) multimers when compared with normal controls. Non-reducing SDS-polyacrylamide gel electrophoresis and western blotting also showed a similar distribution of low, middle and HMW multimers in normal and Gstk1-/- mice. Variation in adiponectin has been correlated with glucose tolerance and with the levels of phosphorylated AMP-kinase but we found similar glucose tolerance and similar levels of phospho 5-AMP-activated protein kinase in normal and Gstk1-/- mice. Consequently, our findings suggest that GSTK1-1 is not absolutely required for adiponectin multimerization in vivo and alternate pathways may be activated in GSTK1-1 deficiency.International Journal of Obesity advance online publication, 17 January 2012; doi:10.1038/ijo.2011.267.
dc.identifier.issn0307-0565
dc.identifier.urihttp://hdl.handle.net/1885/54237
dc.publisherNature Publishing Group
dc.sourceInternational Journal of Obesity
dc.titleThe impact of glutathione transferase kappa deficiency on adiponectin multimerisation in vivo
dc.typeJournal article
local.bibliographicCitation.issue3
local.bibliographicCitation.lastpage33
local.bibliographicCitation.startpage427
local.contributor.affiliationTheodoratos, Angelo, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBlackburn, Anneke, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationCoggan, Marjorie, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationCappello, Jean, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationLarter, Claire, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationMatthaei, Klaus, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBoard, Philip, College of Medicine, Biology and Environment, ANU
local.contributor.authoremailu4036935@anu.edu.au
local.contributor.authoruidTheodoratos, Angelo, u4036935
local.contributor.authoruidBlackburn, Anneke, u4048450
local.contributor.authoruidCoggan, Marjorie, u7400157
local.contributor.authoruidCappello, Jean, u9601131
local.contributor.authoruidLarter, Claire, u4367140
local.contributor.authoruidMatthaei, Klaus, u8200697
local.contributor.authoruidBoard, Philip, u7701651
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.identifier.absfor110107 - Metabolic Medicine
local.identifier.ariespublicationu4020362xPUB295
local.identifier.citationvolume83
local.identifier.doi10.1038/ijo.2011.267
local.identifier.scopusID2-s2.0-84855820608
local.identifier.thomsonID000309649400018
local.identifier.uidSubmittedByu4020362
local.type.statusPublished Version

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