The impact of glutathione transferase kappa deficiency on adiponectin multimerisation in vivo
Date
2012
Authors
Theodoratos, Angelo
Blackburn, Anneke
Coggan, Marjorie
Cappello, Jean
Larter, Claire
Matthaei, Klaus
Board, Philip
Journal Title
Journal ISSN
Volume Title
Publisher
Nature Publishing Group
Abstract
Glutathione transferase Kappa (GSTK1-1) also termed disulfide bond-forming oxidoreductase A-like protein (DsbA-L) has been implicated in the post-translational multimerization of adiponectin and has been negatively correlated with obesity in mice and humans. We investigated adiponectin in Gstk1-/- mice and surprisingly found no difference in the levels of total serum adiponectin or the level of high molecular weight (HMW) multimers when compared with normal controls. Non-reducing SDS-polyacrylamide gel electrophoresis and western blotting also showed a similar distribution of low, middle and HMW multimers in normal and Gstk1-/- mice. Variation in adiponectin has been correlated with glucose tolerance and with the levels of phosphorylated AMP-kinase but we found similar glucose tolerance and similar levels of phospho 5-AMP-activated protein kinase in normal and Gstk1-/- mice. Consequently, our findings suggest that GSTK1-1 is not absolutely required for adiponectin multimerization in vivo and alternate pathways may be activated in GSTK1-1 deficiency.International Journal of Obesity advance online publication, 17 January 2012; doi:10.1038/ijo.2011.267.
Description
Keywords
Citation
Collections
Source
International Journal of Obesity
Type
Journal article
Book Title
Entity type
Access Statement
License Rights
Restricted until
2037-12-31