ANU Open Research Repository will be unavailable 8am-9am on Tuesday 20th August 2024 due to scheduled maintenance.
 

Graded expression of EphA3 in the retina and ephrin-A2 in the superior colliculus during initial development of coarse topography in the wallaby retinocollicular projection

Simple item page
dc.contributor.authorStubbs, J
dc.contributor.authorPalmer, A
dc.contributor.authorVidovic, Maria
dc.contributor.authorMarotte, Lauren
dc.date.accessioned2015-12-13T23:14:44Z
dc.date.issued2000
dc.date.updated2015-12-12T08:40:10Z
dc.description.abstractWe describe the expression of EphA3 and EphA7 receptors and ephrin-A2 ligand in the retina and the superior colliculus during the development of the retinocollicular projection in the marsupial wallaby (Macropus eugenii), using immunoblotting and immunohistochemistry. EphA3 in the retina was in a striking, low central to high peripheral gradient, superimposed on which was a high temporal to low nasal level of expression. This distribution was evident from postnatal day 30, when axons are growing into the colliculus and forming a coarsely organized topographic projection, to postnatal day 65, when axons have arborized in their correct retinotopic positions. EphA7 showed a shallow centroperipheral gradient with no nasotemporal differences in expression. In the superior colliculus no rostrocaudal differences in distribution were detected for either of these receptors. Ephrin-A2 was distributed in a gradient increasing from the rostral to the caudal pole in the superficial layers of the superior colliculus only up to postnatal day 30. Ephrin-A2 was evenly distributed in the retina throughout development of the projection. Expression of EphA3 in the retina increased, while the expression of ephrin-A2 in the colliculus was downregulated over time. The graded expression of EphA3 and ephrin-A2 early in the development of the projection suggests that they play a role in establishment of coarse topography of retinal axons along the rostrocaudal axis of the superior colliculus. However, the gradients were not complementary, meaning that EphA3 alone cannot mediate the repulsive interactions with ephrin-A2 that have been postulated to underlie formation of the topographic map.
dc.identifier.issn0953-816X
dc.identifier.urihttp://hdl.handle.net/1885/88758
dc.publisherBlackwell Publishing Ltd
dc.sourceEuropean Journal of Neuroscience
dc.subjectKeywords: ephrin; animal tissue; article; immunoblotting; immunohistochemistry; marsupial; nonhuman; priority journal; protein determination; protein expression; protein localization; retina; superior colliculus; tissue distribution; Animals; Axons; Down-Regulation EphA7; Mammalian visual system
dc.titleGraded expression of EphA3 in the retina and ephrin-A2 in the superior colliculus during initial development of coarse topography in the wallaby retinocollicular projection
dc.typeJournal article
local.bibliographicCitation.lastpage3636
local.bibliographicCitation.startpage3626
local.contributor.affiliationStubbs, J, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationPalmer, A, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationVidovic, Maria, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationMarotte, Lauren, College of Medicine, Biology and Environment, ANU
local.contributor.authoremailu8501165@anu.edu.au
local.contributor.authoruidStubbs, J, u971845
local.contributor.authoruidPalmer, A, u9707201
local.contributor.authoruidVidovic, Maria, u8501165
local.contributor.authoruidMarotte, Lauren, u7400435
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.absfor060405 - Gene Expression (incl. Microarray and other genome-wide approaches)
local.identifier.ariespublicationMigratedxPub18594
local.identifier.citationvolume12
local.identifier.doi10.1046/j.1460-9568.2000.00251.x
local.identifier.scopusID2-s2.0-0033792194
local.identifier.uidSubmittedByMigrated
local.type.statusPublished Version

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
01_Stubbs_Graded_expression_of_EphA3_in_2000.pdf
Size:
1.47 MB
Format:
Adobe Portable Document Format
Download

Collections

ANU Research Publications

DSpace software copyright © 2002-2024 LYRASIS