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Alphavirus 6K proteins form ion channels

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dc.contributor.authorMelton, Julian
dc.contributor.authorEwart, Gary
dc.contributor.authorWeir, Ron
dc.contributor.authorBoard, Philip
dc.contributor.authorLee, Eva
dc.contributor.authorGage, Peter
dc.date.accessioned2015-12-13T22:25:11Z
dc.date.issued2002
dc.date.updated2015-12-11T08:13:32Z
dc.description.abstractRoss River virus and Barmah Forest virus are Australian arboviruses of the Alphavirus genus. Features of alphavirus infection include an increased permeability of cells to monovalent cations followed by virion budding. Virally encoded ion channels are thought to have a role in these processes. In this paper, the 6K proteins of Ross River virus and Barmah Forest virus are shown to form cation-selective ion channels in planar lipid bilayers. Using a novel purification method, bacterially expressed 6K proteins were inserted into bilayers with a defined orientation (i.e. N-terminal cis, C-terminal trans). Channel activity was reversibly inhibited by antibodies to the N and C termini of 6K protein added to the cis and trans baths, respectively. Channel conductances varied from 40-800 picosiemens, suggesting that the protein is able to form channels with a range of possible oligomerization states.
dc.identifier.issn0021-9258
dc.identifier.urihttp://hdl.handle.net/1885/73116
dc.publisherAmerican Society for Biochemistry and Molecular Biology Inc
dc.sourceJournal of Biological Chemistry
dc.subjectKeywords: Antibodies; Cells; Enzyme inhibition; Mechanical permeability; Positive ions; Viruses; Arboviruses; Ion channels; Biochemistry; ion channel; monovalent cation; virus protein; Alpha virus; article; Barmah Forest virus; conductance; lipid bilayer; membrane
dc.titleAlphavirus 6K proteins form ion channels
dc.typeJournal article
local.bibliographicCitation.issue49.
local.bibliographicCitation.lastpage46931
local.bibliographicCitation.startpage46923
local.contributor.affiliationMelton, Julian, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationEwart, Gary, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationWeir, Ron, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBoard, Philip, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationLee, Eva, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationGage, Peter, College of Medicine, Biology and Environment, ANU
local.contributor.authoremailu4033496@anu.edu.au
local.contributor.authoruidMelton, Julian, u9803533
local.contributor.authoruidEwart, Gary, u4033496
local.contributor.authoruidWeir, Ron, u690062
local.contributor.authoruidBoard, Philip, u7701651
local.contributor.authoruidLee, Eva, u8512358
local.contributor.authoruidGage, Peter, u8404889
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.absfor060110 - Receptors and Membrane Biology
local.identifier.ariespublicationMigratedxPub3561
local.identifier.citationvolume277
local.identifier.doi10.1074/jbc.M207847200
local.identifier.scopusID2-s2.0-0347928844
local.identifier.uidSubmittedByMigrated
local.type.statusPublished Version

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