Asymmetry of glutamine transporters in cultured neural cells

Date

2003

Authors

Henckel, Timo
Broer, Angelika
Wiesinger, H
Lang, Florian
Broer, Stefan

Journal Title

Journal ISSN

Volume Title

Publisher

Elsevier

Abstract

Transfer of glutamine between astrocytes and neurons is an essential part of the glutamate-glutamine cycle in the brain. Transport of glutamine was investigated in primary cultures of astrocytes and neurons and compared to glutamine transport in cell lines with glial and neuronal properties. Glutamine uptake in astrocytes was mainly mediated by general amino acid transporters with properties similar to ASCT2, LAT1, LAT2, SN1 and y+LAT2. In cultured neurons, transport activities were detected consistent with the presence of LAT1, LAT2 and y+LAT2, but the most prominent activity was a novel Na+-dependent glutamine transporter that could be inhibited by D-aspartate. The mRNA for system A isoforms ATA1 and ATA2 was detected in both neurons and astrocytes, but system A activity was only detected in neurons. ASCT2 on the other hand appeared to be astrocyte-specific. The cell lines F98 and 108CC-15, having astroglial and neuronal properties, respectively, expressed sets of glutamine transporters that were unrelated to those of the corresponding primary culture and are thus of limited use as models to study transfer of glutamine between astrocytes and neurons.

Description

Keywords

Keywords: amino acid transporter; dextro aspartic acid; glutamine; messenger RNA; sodium; amino acid transport; animal cell; astrocyte; cell culture; cell specificity; conference paper; controlled study; embryo; glia cell; mouse; nerve cell; newborn; nonhuman; nucl Amino acid transporter; Astrocytes; Glutamate-glutamine cycle; Neurons

Citation

Source

Neurochemistry International

Type

Journal article

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2037-12-31