Quantification of cyclic electron flow in spinach leaf discs
Date
2012-07-01
Authors
Kou, Jiancun
Takahashi, Shunichi
Oguchi, Riichi
Badger, Murray
Chow, Wah Soon
Journal Title
Journal ISSN
Volume Title
Publisher
Zhejiang University Press, Hangzhou and Springer - Verlag GmbH
Abstract
We quantified the photosynthetic cyclic electron flux (CEF) around Photosystem I as the difference between the total electron flux through PS I (ETR1) and the linear electron flux through both photosystems. Both measurements were made in the whole tissue of spinach leaf discs illuminated in the same geometry and in CO2-enriched air to suppress photorespiration. (1) CEF was negligibly small below 300 μmol photons m-2 s-1. Above this irradiance, CEF increased approximately linearly up to the highest irradiance used (1900 μmol photons m-2 s-1). (2) CEF at a fixed irradiance of 980 μmol m-2 s-1 increased by a factor of almost 3 as the temperature was increased from 5°C to 40°C. It did not decline, even when the linear electron flux decreased at high temperatures. (3) Antimycin A, at a high concentration, decreased CEF to about 10% of the control value without affecting the linear electron flux. This method appears to be reliable for quantifyin!
g CEF non-intrusively. By contrast, estimation of the linear electron flux from chlorophyll fluorescence over-estimated CEF in the above treatments.
Description
Keywords
Antimycin A, cyclic, electron, flow, linear, P700, Photosystem I
Citation
Kou, J., Takahashi, S., Oguchi, R., Badger, M. R., Chow, W. S. (2012). Quantification of cyclic electron flow in spinach leaf discs. In T. Kuang, C. Lu, L. Zhang (Eds), Photosynthesis research for food, fuel and future: 15th International Congress on Photosynthesis (pp. 271-274). Beijing: The Institute of Botany, Chinese Academy of Sciences and International Society of Photosynthesis Research
Collections
Source
Advanced Topics in Science and Technology in China: Photosynthesis Research for Food, Fuel and Future: Proceedings of the 15th International Conference on Photosynthesis
Type
Conference paper