Nucleotide sequence diversity of HLA class II genes in Australian Aborigines and populations of Asia-Oceania

dc.contributor.authorGao, Xiaojiang
dc.date.accessioned2015-06-01T05:14:06Z
dc.date.available2015-06-01T05:14:06Z
dc.date.issued1992
dc.description.abstractThe aim of this thesis was to investigate nucleotide sequence diversity of HLA class II genes in Australian Aborigines and indigenous peoples of Asia-Oceania. Nineteen study populations represented eight major ethnic groups including Australian Aborigines, Papua New Guinean highlanders, coastal Melanesians, Polynesians, Micronesians, Javanese, southern and northern Chinese, and a minority group from northwestern China. Using PCR-based technologies, the nucleotide sequence polymorphism in exon 2 DRB1, DRB3, DRB5, DQA1 and DQB1 genes was examined in all these populations. The DPB1 exon 2 polymorphism was examined in Australian Aborigines and a Chinese population. Six novel HLA class II alleles including four DRB1, one DRB5 and one DPBl were discovered in this study by the occurrence of unusual hybridization patterns in the PCR-SSO typing procedure and were confirmed by DNA Sequencing. These new alleles, DRB1*0412, 1408, 1409, 1410, DRB5*0203 and DBP1*2201 have been recognized by the WHO Nomenclature Committee. The nucleotide sequences and the deduced amino acid sequences of the novel class II alleles indicated that multiple molecular mechanisms were involved in generating these alleles including point mutation and hypermutational events of segmental transfer and intra-exonic recombination. In two cases (DRB1*0412 and DRB1*1410), hypermutational events have created unique peptide binding sites which are drastically different from all their putative progenitor molecules. Five of the six novel alleles were found in Australian Aborigines and four novel DRB1 alleles were detected in 45% of the Aboriginal individuals tested. PCR-SSO typing revealed some HLA class II polymorphisms previously difficult or impossible to detect with more traditional typing techniques. Remarkable differences in the V class II HLA allele frequency distributions, especially in the subtypes of major DR antigen groups, were observed between the study populations. Australian Aborigines showed the most divergent class II HLA profile; most of their DRB1 alleles did not overlap with other study populations. PNG highlanders and Javanese were highly homogeneous with quite restricted class II HLA distributions. Other Oceanic populations of Polynesians, Micronesians and coastal Melanesians were each characterized with unique class II HLA distribution but shared common features which indicated their historical ties. Distinctive HLA distributions were observed between Chinese populations from southern and northern China, while the minority group from northwestern China demonstrated a mixed ancestry of both Caucasoids and Orientals. Further information came from the analysis of HLA-DR, -DQ haplotypes. A total of 80 three-locus or four-locus DR-DQ combinations including 16 DR2-related, 12 DR4-related, 11 DR5- related, and 24 DR6-related haplotypes were inferred from the study populations. Haplotype frequencies were used to calculate genetic distances between these populations and to reconstruct population phylogeny, which proved a sensitive indicator of population affinities. The unusual linkage relationships detected in the study populations also had important implications for the understanding of MHC evolution. Knowledge of the nucleotide sequence polymorphism of HLA class II genes in general populations has fundamental importance in HLA-related clinical investigations. The apparent lack of susceptible alleles in the HLA gene pool of native Australians and Pacific islanders, or the high frequency of protective alleles, might partly explain the extremely low incidence of autoimmune diseases in these populations.en_AU
dc.identifier.otherb18583416
dc.identifier.urihttp://hdl.handle.net/1885/13687
dc.language.isoenen_AU
dc.subjectJohn Curtin School of Medical Researchen_AU
dc.subjectnucleotide sequence diversityen_AU
dc.subjectHLA class II genersen_AU
dc.subjectAustralian Aboriginesen_AU
dc.subjectindigenous peoplesen_AU
dc.subjectAsia-Oceaniaen_AU
dc.titleNucleotide sequence diversity of HLA class II genes in Australian Aborigines and populations of Asia-Oceaniaen_AU
dc.typeThesis (PhD)en_AU
dcterms.valid1992en_AU
local.contributor.affiliationJohn Curtin School of Medical Research, The Australian National Universityen_AU
local.contributor.supervisorSerjeantson, Prof S W
local.identifier.doi10.25911/5d70f1cfe8152
local.mintdoimint
local.type.degreeDoctor of Philosophy (PhD)en_AU

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